4N6B
Soybean Serine Acetyltransferase Complexed with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.077, 99.181, 120.256 |
| Unit cell angles | 90.00, 117.23, 90.00 |
Refinement procedure
| Resolution | 37.956 - 3.005 |
| R-factor | 0.2169 |
| Rwork | 0.214 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.952 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.960 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 33560 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 12% ethanol, 100 mM Tris (pH 7.8), 200 mM MgCl2, 1 mM Tris(2-carboxyethyl)phosphine hydrochloride, and 10 mM CoA, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
