4N5U

Crystal structure of the 4th FN3 domain of human Protein Tyrosine phosphatase, receptor type F [PSI-NYSGRC-006240]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 21 21 21
Unit cell lengths	36.011, 39.677, 68.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.337 - 1.456
R-factor	0.1641
Rwork	0.162
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2edx
RMSD bond length	0.009
RMSD bond angle	1.233
Data reduction software	XDS
Data scaling software	SCALA (0.1.29)
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.337
High resolution limit [Å]	1.456
Number of reflections	16720
Completeness [%]	94.0
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	298	Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 5% glycerol, Reservoir (0.2M Lithium Sulfate, 0.1M CAPS, 1.2M NaH2PO4/0.8M K2HPO4), Cryoprotection (2M Li2SO4), VAPOR DIFFUSION, SITTING DROP, temperature 298K