4N5Q

Crystal structure of the N-terminal ankyrin repeat domain of TRPV3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	61.518, 78.557, 82.975
Unit cell angles	90.00, 93.30, 90.00

Refinement procedure

Resolution	41.400 - 1.946
R-factor	0.1705
Rwork	0.169
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.575
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.400
High resolution limit [Å]	1.946
Number of reflections	57543
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	2.4M sodium formate, 0.1M BIS-TRIS propane, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K