4N5C
Crystal structure of Ypp1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.545, 136.650, 154.125 |
| Unit cell angles | 76.69, 85.67, 72.74 |
Refinement procedure
| Resolution | 25.000 - 3.250 |
| R-factor | 0.2404 |
| Rwork | 0.240 |
| R-free | 0.27720 |
| Structure solution method | MR_SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.112 | 0.582 |
| Number of reflections | 114807 | |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 Bis-Tris propane [pH 7.5], 0.2 M sodium bromide, 19% PEG 3,350, and 20 mM taurine, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
