4N4R
Structure basis of lipopolysaccharide biogenesis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2013-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97887, 0.97835, 0.98181, 0.97750 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 173.430, 76.082, 213.596 |
| Unit cell angles | 90.00, 111.52, 90.00 |
Refinement procedure
| Resolution | 107.490 - 2.800 |
| R-factor | 0.28638 |
| Rwork | 0.285 |
| R-free | 0.30508 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.718 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.490 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.154 | 0.904 |
| Number of reflections | 64252 | |
| <I/σ(I)> | 1.6 | 1.6 |
| Completeness [%] | 9.7 | 94 |
| Redundancy | 5.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M zinc acetate, 0.1M sodium cacodylate, 18% PEG8000 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
