4N4Q
Crystal Structure of N-acetylneuraminate lyase from Mycoplasma synoviae, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.89997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.225, 142.441, 80.792 |
| Unit cell angles | 90.00, 108.27, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2413 |
| Rwork | 0.239 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n4p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 76.718 | 47.480 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.330 | 2.000 |
| Rmerge | 0.063 | 0.499 | |
| Total number of observations | 9537 | 41164 | |
| Number of reflections | 85882 | ||
| <I/σ(I)> | 6.7 | 6.4 | 1.5 |
| Completeness [%] | 99.0 | 99.1 | 97.6 |
| Redundancy | 3.5 | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 50 mM MES, pH 6.0, 50 % MPD, vapor diffusion, sitting drop, temperature 277K |
