4N4H
Crystal structure of the Bromo-PWWP of the mouse zinc finger MYND-type containing 11 isoform alpha in complex with histone H3.1K36me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0076 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 64.358, 64.358, 122.059 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.482 - 2.302 |
R-factor | 0.2105 |
Rwork | 0.206 |
R-free | 0.26310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n4g |
RMSD bond length | 0.003 |
RMSD bond angle | 0.692 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.099 | 0.048 | 0.769 |
Number of reflections | 11677 | ||
<I/σ(I)> | 12.9 | ||
Completeness [%] | 97.4 | 98.2 | 100 |
Redundancy | 11.4 | 9.9 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 289 | 25% (w/v) polyethylene glycol 4000, 0.1M Tris-HCl, pH 8.3, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |