4N3N

Crystal structure of eukaryotic translation initiation factor eIF5B (517-1116) from Chaetomium thermophilum, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826580
Spacegroup name	P 32 2 1
Unit cell lengths	111.470, 111.470, 115.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.683 - 2.752
R-factor	0.195
Rwork	0.193
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g7r
RMSD bond length	0.004
RMSD bond angle	0.825
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.680		2.850
High resolution limit [Å]	2.750	50.000	2.750
Rmerge	0.036		0.515
Number of reflections	21910		2198
<I/σ(I)>	33		3.76
Completeness [%]	99.9		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	12% PEG 20000, 10 mM Na-lactate, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K