4N30
Crystal structure of Pseudomonas aeruginosa DsbA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.797, 59.380, 59.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.050 - 1.300 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.21969 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.283 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.050 | 1.331 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 38970 | |
| Completeness [%] | 98.5 | 96.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1mM Bis-Tris, pH 5.5, 1.5M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
