4N2Z
Crystal Structure of the alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina in complex with cellotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Nova high-flux-micro-focus sealed tube |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-02 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.140, 66.790, 60.360 |
| Unit cell angles | 90.00, 117.33, 90.00 |
Refinement procedure
| Resolution | 14.840 - 1.800 |
| R-factor | 0.13309 |
| Rwork | 0.131 |
| R-free | 0.16963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n1i |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.078 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 32128 | |
| <I/σ(I)> | 22 | 2.51 |
| Completeness [%] | 97.6 | 87.2 |
| Redundancy | 12.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 285 | 25% PEG 4000, 0.2M Calcium chloride, 0.1M Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
