4N2K
Crystal structure of Protein Arginine Deiminase 2 (Q350A, 0 mM Ca2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Detector technology | CCD |
Collection date | 2013-06-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.242, 51.940, 75.969 |
Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
Resolution | 40.582 - 1.570 |
R-factor | 0.1794 |
Rwork | 0.178 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n20 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.244 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.582 | |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.071 | 0.489 |
Number of reflections | 103006 | |
<I/σ(I)> | 26.8 | 2 |
Completeness [%] | 96.7 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 10-20% MPD, 50 mM MES, pH 5.6, 0.12 M sodium acetate, VAPOR DIFFUSION, SITTING DROP |