4N27
X-ray structure of Brucella abortus RicA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.430, 57.440, 127.610 |
| Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
| Resolution | 63.770 - 2.730 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.393 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.770 | 2.798 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 39871 | |
| Completeness [%] | 100.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 100 mM Sodium Acetate, pH 4.6, 25 % (w/v) PEG 1000, 200 mM NaCl, 30 mM Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
