4N26
Crystal structure of Protein Arginine Deiminase 2 (500 uM Ca2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2004-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.278, 51.675, 76.099 |
Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
Resolution | 33.995 - 1.943 |
R-factor | 0.1721 |
Rwork | 0.170 |
R-free | 0.22230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n25 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.243 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.995 | |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.087 | 0.435 |
Number of reflections | 52719 | |
<I/σ(I)> | 14.7 | 2.1 |
Completeness [%] | 93.8 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 10-20% MPD, 50 mM MES, pH 5.6, 0.12 M sodium acetate, VAPOR DIFFUSION, SITTING DROP |