4N1A
Thermomonospora curvata EccC (ATPases 2 and 3) in complex with a signal sequence peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-01-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 216.150, 216.150, 186.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.150 - 3.240 |
Rwork | 0.226 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 152.600 |
High resolution limit [Å] | 3.240 |
Number of reflections | 85798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | Citric Acid, pH 5.2, 1.28M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |