4N0P
Crystal structure of a pilus assembly protein CpaE (CC_2943) from Caulobacter crescentus CB15 at 1.75 A resolution (PSI Community Target, Shapiro)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.600, 112.780, 73.880 |
| Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
| Resolution | 47.271 - 1.750 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20540 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (December 29, 2011) |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.271 | 47.271 | 1.800 |
| High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
| Rmerge | 0.057 | 0.033 | 0.797 |
| Number of reflections | 105477 | 1238 | 7796 |
| <I/σ(I)> | 13.4 | 35.5 | 1.7 |
| Completeness [%] | 97.8 | 97.9 | 97.7 |
| Redundancy | 3.8 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 25% PEG 1000; 0.2M Ammonium Acetate, 0.1M TRIS pH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 295K |
| 2 |
