4N0N

Crystal structure of Arterivirus nonstructural protein 10 (helicase)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	130
Detector technology	CCD
Collection date	2010-10-14
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	89.763, 91.041, 57.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.19594
Rwork	0.195
R-free	0.22384
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.168
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	32746
Completeness [%]	97.8	86.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	299	2 M (NH4)2SO4, 0.1 M HEPES, 25 mM KCl and 20% ethylene glycol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 299K
1	VAPOR DIFFUSION, HANGING DROP	7.1	299	2 M (NH4)2SO4, 0.1 M HEPES, 25 mM KCl and 20% ethylene glycol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 299K