4N06
Crystal structure of Cas1 from Archaeoglobus fulgidus and its nucleolytic activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9897 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.886, 41.929, 125.293 |
| Unit cell angles | 90.00, 98.03, 90.00 |
Refinement procedure
| Resolution | 65.116 - 2.400 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.24130 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.116 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 28962 | |
| Completeness [%] | 99.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 30%(w/v) Polyethyleneglycol Monomethylether 550, 0.1M Bicine, 0.1M Sodium Chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
