4N05
The crystal structure of R43A mutant putative ryanodine receptor from Bacteroides Thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | MOLECULAR REPLACEMENT |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.362, 88.673, 74.370 |
| Unit cell angles | 90.00, 111.00, 90.00 |
Refinement procedure
| Resolution | 42.120 - 2.605 |
| R-factor | 0.1875 |
| Rwork | 0.184 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 26789 | |
| <I/σ(I)> | 11.9 | 4 |
| Completeness [%] | 97.2 | 96.2 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 1.5M NA CITRATE, 0.1M BISTRIS 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
