4MZR

Crystal structure of a polypeptide p53 mutant bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	85
Detector technology	CCD
Collection date	2011-06-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 21 21 2
Unit cell lengths	163.298, 169.764, 55.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.000 - 2.900
R-factor	0.234
Rwork	0.234
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q05
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	53.000
High resolution limit [Å]	2.900
Number of reflections	4796
Completeness [%]	94.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	0.2M potassium sodium tartrate tetrahydrate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K