4MZP
MazF from S. aureus crystal form III, C2221, 2.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0394 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.881, 92.636, 222.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.200 - 2.702 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mzm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 ((Phaser)) |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.200 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.093 |
| Number of reflections | 25526 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 98.0 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.0 M (NH4)2SO4, 0.1 M NAAC pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
