4MZJ
Crystal Structure of MTIP from Plasmodium falciparum in complex with pGly[801,805], a stapled myoA tail peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.380, 53.980, 75.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.800 - 1.474 |
Rwork | 0.180 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aom |
RMSD bond length | 0.005 |
RMSD bond angle | 0.981 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.800 | 37.810 | 1.510 |
High resolution limit [Å] | 1.470 | 6.590 | 1.470 |
Rmerge | 0.045 | 0.025 | 0.860 |
Number of reflections | 26460 | ||
<I/σ(I)> | 33.4 | 93.9 | 2.9 |
Completeness [%] | 99.6 | 99.7 | 95.6 |
Redundancy | 11.9 | 9.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Reservoir solution: 20% PEG 3350, 0.2M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |