4MZD
High resolution crystal structure of the nisin leader peptidase NisP from Lactococcus lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.890, 44.973, 74.651 |
| Unit cell angles | 90.00, 106.82, 90.00 |
Refinement procedure
| Resolution | 26.947 - 1.100 |
| R-factor | 0.1525 |
| Rwork | 0.152 |
| R-free | 0.16820 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.459 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.078 | 0.448 |
| Number of reflections | 128500 | |
| <I/σ(I)> | 38.7 | 2.4 |
| Completeness [%] | 99.9 | 95.8 |
| Redundancy | 6.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 24-32% 1,4-dioxane, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
