4MZ2
Crystal structure of the voltage-gated sodium channel beta 4 subunit extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9749 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 43.445, 43.445, 108.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.058 - 1.722 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1neu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.189 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.770 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.060 | 0.701 |
| Number of reflections | 13164 | |
| <I/σ(I)> | 21.4 | 2.9 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 10.4 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, 15% PEG20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
