4MYU

Crystal structure of elongation factor G mutant(EFG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2012-10-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	78.282, 88.235, 115.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.060 - 3.004
R-factor	0.1958
Rwork	0.187
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m1k
RMSD bond length	0.008
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	16397
Completeness [%]	99.9	100
Redundancy	8.2	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	5mM Tris-HCl (pH 7.6), 10mM MgCl2, 5mM GDP, 20-23% PEG 8000, 0.1M Tris-Cl (PH 7.5-7.6), VAPOR DIFFUSION, HANGING DROP, temperature 289K