4MYM
Crystal structure of a glyoxalase/ bleomycin resistance protein/ dioxygenase from Nocardioides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.660, 83.589, 102.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.030 - 1.900 |
| R-factor | 0.18798 |
| Rwork | 0.186 |
| R-free | 0.21722 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.455 |
| Number of reflections | 14825 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.8 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350, 0.1M Bis-Tris, 0.2M NaCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
