4MYK
Crystal structure of Trypanosoma cruzi formiminoglutamase (oxidized) with Mn2+2 at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 129.729, 129.729, 42.482 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.126 - 1.518 |
| R-factor | 0.147 |
| Rwork | 0.146 |
| R-free | 0.16210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4myf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER ((CCP4)) |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.077 | 0.422 |
| Number of reflections | 40463 | |
| <I/σ(I)> | 14.367 | 2.732 |
| Completeness [%] | 98.4 | 95.1 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | An apo-T. cruzi formiminoglutamase (oxidized) crystal was soaked in 20 mM MnCl2, 0.1 M sodium malonate (pH8.5), 27% PEG 3350 for 12 hours, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
