4MY7
frog M ferritin iron-loaded under anaerobic environment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976, 1.722, 1.758 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.830, 183.830, 183.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.110 - 1.480 |
| R-factor | 0.17548 |
| Rwork | 0.174 |
| R-free | 0.19504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.960 | 45.960 | 1.560 |
| High resolution limit [Å] | 1.480 | 4.680 | 1.480 |
| Rmerge | 0.086 | 0.063 | 0.420 |
| Number of reflections | 55514 | ||
| <I/σ(I)> | 15.4 | 29.7 | 4.7 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 9.7 | 8.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.6-2M magnesium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
