4MWH
Crystal structure of scCK2 alpha in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.773, 69.773, 168.514 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.910 - 2.090 |
| R-factor | 0.19866 |
| Rwork | 0.196 |
| R-free | 0.24098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 29510 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 287 | 2mM ATP, 4mM magnesium chloride, 200mM ammonium sulfate, 20% w/v polyethylene glycol 8000, 100mM MES, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
