4MVZ
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.525, 177.825, 131.355 |
| Unit cell angles | 90.00, 132.57, 90.00 |
Refinement procedure
| Resolution | 29.900 - 3.300 |
| R-factor | 0.24395 |
| Rwork | 0.242 |
| R-free | 0.28007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.572 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.095 | 0.595 |
| Number of reflections | 47359 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 95.9 | 97.3 |
| Redundancy | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
