4MVU
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.75 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.765, 177.685, 131.205 |
Unit cell angles | 90.00, 132.64, 90.00 |
Refinement procedure
Resolution | 29.890 - 3.198 |
R-factor | 0.23802 |
Rwork | 0.237 |
R-free | 0.26254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rvy |
RMSD bond length | 0.013 |
RMSD bond angle | 1.584 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.370 |
High resolution limit [Å] | 3.198 | 3.198 |
Rmerge | 0.076 | 0.500 |
Number of reflections | 46122 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 93.4 | 100 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |