4MUO
The TrpD2 enzyme from E.coli: YbiB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.460, 67.440, 105.110 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.940 |
| R-factor | 0.1801 |
| Rwork | 0.179 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.098 | 2.000 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 46153 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 97.9 | 92.9 |
| Redundancy | 3.75 | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 30% PEG 600, 100 mM sodium citrate, pH 5.5, VAPOR DIFFUSION, temperature 298K |
