4MTX
Structure of the ERS1 dimerization and histidine phosphotransfer domain from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9919 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.911, 69.193, 108.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.700 - 2.150 |
| R-factor | 0.2009 |
| Rwork | 0.198 |
| R-free | 0.25060 |
| Structure solution method | UV-RIP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: dev_1092)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 27137 | |
| Completeness [%] | 98.1 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.18 M L-proline, 0.1 M HEPES pH 7.5, 9% PEG 3350, 0.12 M sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
