4MTV
Crystal structure of the complex of Buffalo Signalling Glycoprotein with pentasaccharide at 2.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.870, 66.499, 107.126 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.500 - 2.800 |
| R-factor | 0.1837 |
| Rwork | 0.181 |
| R-free | 0.22902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o9o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.509 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 10772 | |
| <I/σ(I)> | 4.5 | 1.8 |
| Completeness [%] | 92.4 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 25mM Tris, 50mM NaCl, 20% ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
