4MTO
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.75 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 176.635, 177.655, 130.600 |
Unit cell angles | 90.00, 132.53, 90.00 |
Refinement procedure
Resolution | 29.990 - 3.402 |
R-factor | 0.26144 |
Rwork | 0.260 |
R-free | 0.29741 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.679 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.125 | |
Number of reflections | 36840 | |
Completeness [%] | 90.7 | 93.9 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | 0.1M Na-citrate,pH 4.75, 2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |