4MTN
Crystal structure of transcription termination factor NusA from Planctomyces limnophilus DSM 3776
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.026, 54.065, 62.859 |
| Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
| Resolution | 38.677 - 2.579 |
| R-factor | 0.2235 |
| Rwork | 0.222 |
| R-free | 0.25090 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.575 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.092 | 0.476 |
| Number of reflections | 16040 | |
| <I/σ(I)> | 23 | 2.1 |
| Completeness [%] | 99.6 | 96.6 |
| Redundancy | 4.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M Ammonium sulfate, 22% PEG 4000, 0.1M sodium acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
