4MTL
Human Methyltransferase-Like Protein 21C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97856 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 103.554, 43.247, 119.454 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 40.640 - 1.650 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.19550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lec |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.090 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.054 | 1.097 |
| Total number of observations | 11457 | |
| Number of reflections | 62809 | |
| <I/σ(I)> | 13.2 | 1.2 |
| Completeness [%] | 99.3 | 98.1 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 30% PEG3350, 0.2 M sodium chloride, 0.1 M HEPES, 0.005 M SAH, pH 7.5, VAPOR DIFFUSION, temperature 291K |
