4MTD
Zinc Uptake Regulator Complexed With Zinc AND DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2011-02-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.324, 80.470, 98.770 |
| Unit cell angles | 90.00, 120.15, 90.00 |
Refinement procedure
| Resolution | 36.340 - 2.500 |
| R-factor | 0.23602 |
| Rwork | 0.234 |
| R-free | 0.27125 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.046 | 0.290 |
| Number of reflections | 45429 | |
| <I/σ(I)> | 19.4 | 5 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 1.5 M Ammonium Sulfate, 0.1 M Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
