4MT5
Crystal structure of Mub-RV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-27 |
| Detector | Pilatus 6M-F |
| Wavelength(s) | 0.9205 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 31.400, 271.730, 142.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.230 - 2.600 |
| R-factor | 0.2137 |
| Rwork | 0.211 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.230 | 49.230 | 2.670 |
| High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
| Rmerge | 0.032 | 0.360 | |
| Total number of observations | 1163 | 8694 | |
| Number of reflections | 19616 | ||
| <I/σ(I)> | 16.6 | 18.9 | 2 |
| Completeness [%] | 99.8 | 95.4 | 99.9 |
| Redundancy | 6.1 | 4.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M ammonium acetate, 24 % (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
