4MS8
42F3 TCR pCPB9/H-2Ld Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 102 |
| Detector technology | PIXEL |
| Collection date | 2010-01-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.260, 60.620, 70.100 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 34.854 - 1.922 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.575 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.710 | 2.030 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.043 | |
| Number of reflections | 52108 | |
| Completeness [%] | 93.6 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 4000, 100mM TRIS pH8, 200mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
