4MR0
Crystal structure of PfbA, a surface adhesin of Streptococcus pneumoniae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-10-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97927, 0.97942, 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.590, 126.080, 63.480 |
| Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.22 |
| Rwork | 0.204 |
| R-free | 0.22230 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.860 | 37.860 | 2.000 |
| High resolution limit [Å] | 1.850 | 6.010 | 1.850 |
| Number of reflections | 132827 | ||
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30-35% PEG3350, 0.1M Sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
