4MQV

Crystal complex of Rpa32c and Smarcal1 N-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 6 2 2
Unit cell lengths	149.089, 149.089, 46.055
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.590 - 1.950
R-factor	0.193
Rwork	0.192
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.551
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0087)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.950	1.950
Number of reflections	21338
Completeness [%]	94.8	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	25% PEG 4000, 0.2M Sodium Acetate, pH 8.0, VAPOR DIFFUSION, temperature 298K