4MPZ

Crystal structure of TCP10c domain of Drosophila melanogaster Sas-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	69.344, 69.344, 112.337
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.022 - 2.701
R-factor	0.2468
Rwork	0.244
R-free	0.27590
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.659
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.092
Number of reflections	8481
Completeness [%]	100.0	100	100
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	10% PEG 3350, 0.2M NH4Ac, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K