4MPS
Crystal structure of rat Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1), Northeast Structural Genomics Consortium Target RnR367A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-29 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97905 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.720, 49.754, 86.063 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 46.670 - 2.400 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.119 | 0.486 |
| Number of reflections | 43837 | |
| <I/σ(I)> | 10.71 | 2.46 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 291 | 0.1M Lithium Bromide, 0.1M Tris, pH 8, 40% (v/w) PEG 1000., micro batch, temperature 291K |
