4MOP
Pyranose 2-oxidase V546C mutant with 3-fluorinated galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.971, 102.980, 137.654 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 48.230 - 2.300 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.757 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.230 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 123398 | |
| <I/σ(I)> | 8.7 | 1.6 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1 M MES, 50 mM MgCl2, 10% (w/v) monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
