4MON

ORTHORHOMBIC MONELLIN

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1985-09-01
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	54.000, 112.420, 40.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.15 *
Rwork	0.150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mon
RMSD bond length	0.013
RMSD bond angle	0.033
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	MERLOT
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.300
Rmerge	0.066 *
Total number of observations	36281 *
Number of reflections	10602
Completeness [%]	90.9
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2		5-10 MG/ML PROTEIN DISSOLVED IN 100 MM SODIUM PHOSPHATE BUFFER, PH 7.2, DIFFUSED AGAINST 20 W/V % ETHANOL. CRYSTALS UNSTABLE- CROSSLINKED WITH 0.015% GLUTARALDEHYDE FOR 1-1.5 HOURS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ethanol	20 (%(v/v))
2	1	drop	protein	5-10 (mg)
3	1	reservoir	sodium phosphate	100 (mM)	pH7.2