4MOK
Pyranose 2-oxidase H167A mutant soaked with 3-fluorinated galactose (not bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.875, 102.295, 136.957 |
| Unit cell angles | 90.00, 90.78, 90.00 |
Refinement procedure
| Resolution | 47.920 - 1.900 |
| R-factor | 0.17037 |
| Rwork | 0.170 |
| R-free | 0.21382 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.901 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 216233 | |
| <I/σ(I)> | 13.2 | 2.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1 M MES, 50 mM MgCl2, 10% (w/v) monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
