4MO4
Crystal structure of AnmK bound to AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-22 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.657, 70.823, 91.211 |
| Unit cell angles | 106.50, 104.93, 98.26 |
Refinement procedure
| Resolution | 44.856 - 1.670 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qbw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.860 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.050 | 0.593 |
| Number of reflections | 151753 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 97.2 | 95.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 298 | 26% PEG4000, 0.2 M magnesium chloride, 0.1 M Tris, pH 9.0, VAPOR DIFFUSION, temperature 298K |
