4MO1
Crystal structure of antitermination protein Q from bacteriophage lambda. Northeast Structural Genomics Consortium target OR18A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 32 |
| Unit cell lengths | 54.474, 54.474, 109.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.348 - 2.099 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.099 | 2.100 |
| Rmerge | 0.082 | 0.666 |
| Number of reflections | 42458 | |
| <I/σ(I)> | 16.1 | 1.3 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 10 | 277 | 12% PEG 20000, 0.1M ammonium bromide, 0.1M CAPS, pH 10.0, Microbatch crystallization under oil, temperature 277K |
