4MNA
Crystal structure of the free FLS2 ectodomains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-06-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 189.542, 189.542, 117.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.728 - 3.998 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rgz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.998 |
| Number of reflections | 20722 |
| Completeness [%] | 99.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M sodium cacodylate trihydrate pH 5.0, 0.11M Zinc acetate dihydrate, 14.25% (v/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
