4MN8

Crystal structure of flg22 in complex with the FLS2 and BAK1 ectodomains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	298
Detector technology	CCD
Collection date	2012-07-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	108.865, 113.683, 164.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.962 - 3.062
R-factor	0.2268
Rwork	0.224
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rgz
RMSD bond length	0.004
RMSD bond angle	1.028
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.050
Number of reflections	38653
Completeness [%]	99.2
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.1M citirc acid pH 4.0, 2.5M ammonium sulfate, 8% (v/v) PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K